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pstat3 y705  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc pstat3 y705
    Pstat3 Y705, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pstat3+y705/pmc13053052-70-3-19
    Average 86 stars, based on 1 article reviews
    pstat3 y705 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Expressing:

    Article Title: AXL mediates mast cell survival and resistance to tyrosine kinase inhibitors in mastocytosis
    Article Snippet: Immunoblotting was performed on protein extracts from ROSA and HMC1.2 cells, with or without IFN stimulation, using anti-AXL (8661, Cell Signaling). .. ROSA KIT D816V cells expressing AXL WT-tdTomato, AXL L197M-tdTomato, or control were analyzed for the expression of STAT3 (9139, Cell Signaling), pSTAT3 (Y705) (9145, Cell Signaling), STAT5 (25656, Cell Signaling), pSTAT5 (Y694) (9359, Cell Signaling), FAK (3285, Cell Signaling), pFAK (Y397) (8556, Cell Signaling), p38α (sc-81621, Santa cruz), p-p38 (sc-7973, Santa cruz). .. The chemiluminescence signals were detected using ChemiDoc Imaging Systems (Bio-Rad).

    Control:

    Article Title: AXL mediates mast cell survival and resistance to tyrosine kinase inhibitors in mastocytosis
    Article Snippet: Immunoblotting was performed on protein extracts from ROSA and HMC1.2 cells, with or without IFN stimulation, using anti-AXL (8661, Cell Signaling). .. ROSA KIT D816V cells expressing AXL WT-tdTomato, AXL L197M-tdTomato, or control were analyzed for the expression of STAT3 (9139, Cell Signaling), pSTAT3 (Y705) (9145, Cell Signaling), STAT5 (25656, Cell Signaling), pSTAT5 (Y694) (9359, Cell Signaling), FAK (3285, Cell Signaling), pFAK (Y397) (8556, Cell Signaling), p38α (sc-81621, Santa cruz), p-p38 (sc-7973, Santa cruz). .. The chemiluminescence signals were detected using ChemiDoc Imaging Systems (Bio-Rad).

    Cell-Signaling:

    Article Title: Vincristine impairs musculoskeletal development in pediatric mice
    Article Snippet: Blots were then visualized with Odyssey Infrared Imaging System (LI‐COR Biosciences, Lincoln, NE, USA). .. Antibodies (from Cell Signaling Technologies) used were pSTAT3 Y705 (#9145), STAT3 (#8768), pAKT Ser473 (#9271), AKT (#9272), and Ubiquitin (#3933). .. Optical density measurements were taken using the Image Lab Software (Bio‐Rad, Hercules, CA, USA).

    Ubiquitin Proteomics:

    Article Title: Vincristine impairs musculoskeletal development in pediatric mice
    Article Snippet: Blots were then visualized with Odyssey Infrared Imaging System (LI‐COR Biosciences, Lincoln, NE, USA). .. Antibodies (from Cell Signaling Technologies) used were pSTAT3 Y705 (#9145), STAT3 (#8768), pAKT Ser473 (#9271), AKT (#9272), and Ubiquitin (#3933). .. Optical density measurements were taken using the Image Lab Software (Bio‐Rad, Hercules, CA, USA).

    other:

    Article Title: Aberrant expression of the testis kinase TSSK6activates FAK–STAT3 signaling to promote tumorigenic growth
    Article Snippet: Antibodies used: TSSK6 (sc-514076, Santa Cruz) (1:500 IB), STAT3 (sc-8019, Santa Cruz (1:1000 IB), STAT3 (9139, CST)(1:1000 IB, 1:300 IF), pSTAT3 (Ser727) (9134, CST)(1:1000 IB), pSTAT3 (Y705) (9131, CST), Vinculin (sc-73614, Santa Cruz) (1:2000 IB), Beta-Tubulin (2128, CST) (1:5000), V5-tag (anti-rabbit,13202, CST) (1:5000 IB), V5-tag (anti-mouse, 46-0705, Invitrogen)(1:2000 IB), PLEKHA7 (PA5-85686, Invitrogen) (1:1000 IB), MEF2C (ab227085, Abcam)(1:5000 IB), pFAK (Y397) (44-624G, Invitrogen) (1:1000 IB, 1:200 IF), FAK (3286, CST)(1:1000 IB).

    Incubation:

    Article Title: KDM3A drives NSCLC proliferation and metastasis via H3K9 demethylation, EMT activation, and MMP-9 upregulation
    Article Snippet: .. Membranes were then incubated overnight at 4 ∘ C with antibodies against KDM3A (GeneTex, 54313, 1:1000), KDM3B (Proteintech, 19915-1-AP, 1:1000), H3K9me2 (Cell Signaling Technology, 4658T, 1:2000), β-actin (Santa Cruz Biotechnology, sc-47778, 1:1000), matrix metalloproteinase-9 (MMP-9) (Santa Cruz Biotechnology, sc-393859, 1:1000), E-cadherin (Santa Cruz Biotechnology, sc-8426, 1:1000), Vimentin (Santa Cruz Biotechnology, sc-6260, 1:1000), FAK (Cell Signaling Technology, 3285S, 1:1000), pFAK (Tyr397) (Cell Signaling Technology, 3283, 1:1000), STAT3 (Cell Signaling Technology, 4904, 1:1000), pSTAT3 (Y705) (Cell Signaling Technology, 9131, 1:1000), and GAPDH (Proteintech, HRP-60004, 1:5000). .. Membranes were then washed three times with 1 × TBST and incubated for one hour at room temperature with HRP-labeled secondary antibodies (ab205718 for anti-Rabbit, ab20571 for anti-Mouse, Abcam, 1:10000).



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    CSPG4 blockade reduces TcdB‐induced <t>pSTAT3</t> nuclear translocation in enteric glial cells. (A) Representative photomicrographs of pSTAT3 (green) immunostaining, phalloidin (red) as a cytoplasm staining, and DAPI (blue) as a nuclear staining in enteric glial cells exposed to TcdA (50 ng/mL) or TcdB (1 ng/mL) at 18 h incubation in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). Scale bars: 50 μm. (B) Percentage of enteric glial cells ( n = 5, mean ± SEM) with positive nuclear pSTAT3 staining at 18 h of incubation with TcdA and TcdB in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). One‐way ANOVA followed by the Tukey test.
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    CSPG4 blockade reduces TcdB‐induced <t>pSTAT3</t> nuclear translocation in enteric glial cells. (A) Representative photomicrographs of pSTAT3 (green) immunostaining, phalloidin (red) as a cytoplasm staining, and DAPI (blue) as a nuclear staining in enteric glial cells exposed to TcdA (50 ng/mL) or TcdB (1 ng/mL) at 18 h incubation in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). Scale bars: 50 μm. (B) Percentage of enteric glial cells ( n = 5, mean ± SEM) with positive nuclear pSTAT3 staining at 18 h of incubation with TcdA and TcdB in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). One‐way ANOVA followed by the Tukey test.
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    CSPG4 blockade reduces TcdB‐induced <t>pSTAT3</t> nuclear translocation in enteric glial cells. (A) Representative photomicrographs of pSTAT3 (green) immunostaining, phalloidin (red) as a cytoplasm staining, and DAPI (blue) as a nuclear staining in enteric glial cells exposed to TcdA (50 ng/mL) or TcdB (1 ng/mL) at 18 h incubation in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). Scale bars: 50 μm. (B) Percentage of enteric glial cells ( n = 5, mean ± SEM) with positive nuclear pSTAT3 staining at 18 h of incubation with TcdA and TcdB in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). One‐way ANOVA followed by the Tukey test.
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    Image Search Results


    CSPG4 blockade reduces TcdB‐induced pSTAT3 nuclear translocation in enteric glial cells. (A) Representative photomicrographs of pSTAT3 (green) immunostaining, phalloidin (red) as a cytoplasm staining, and DAPI (blue) as a nuclear staining in enteric glial cells exposed to TcdA (50 ng/mL) or TcdB (1 ng/mL) at 18 h incubation in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). Scale bars: 50 μm. (B) Percentage of enteric glial cells ( n = 5, mean ± SEM) with positive nuclear pSTAT3 staining at 18 h of incubation with TcdA and TcdB in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). One‐way ANOVA followed by the Tukey test.

    Journal: The FASEB Journal

    Article Title: CSPG4 Mediates Inflammatory, Cell Death, and Senescence Responses in Enteric Glia Exposed to Clostridioides difficile Toxins

    doi: 10.1096/fj.202600333R

    Figure Lengend Snippet: CSPG4 blockade reduces TcdB‐induced pSTAT3 nuclear translocation in enteric glial cells. (A) Representative photomicrographs of pSTAT3 (green) immunostaining, phalloidin (red) as a cytoplasm staining, and DAPI (blue) as a nuclear staining in enteric glial cells exposed to TcdA (50 ng/mL) or TcdB (1 ng/mL) at 18 h incubation in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). Scale bars: 50 μm. (B) Percentage of enteric glial cells ( n = 5, mean ± SEM) with positive nuclear pSTAT3 staining at 18 h of incubation with TcdA and TcdB in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). One‐way ANOVA followed by the Tukey test.

    Article Snippet: After washing, cells were incubated overnight at 4°C with primary antibodies against CSPG4 (Abcam, ab275024, 1:400), nectin‐3 (PVRL3, Invitrogen, PA5‐51095, 1:200), LRP1 (Abcam, ab92544, 1:100), NFκB p65 (Santa Cruz Biotechnology, sc‐372, 1:100), or pSTAT3 (R&D Systems, AF4607, 1:100).

    Techniques: Translocation Assay, Immunostaining, Staining, Incubation, Control